BACKGROUND: Traditional medicinal systems like Ayurveda and Indian folk medicine have used Honey, Ghee, Glycyrrhiza glabra, and Nerium indicumeffectively for treating wounds. The known result of these medications is faster healing. However, the mechanism of actions at the tissuelevel, the biochemical and molecular mechanisms of healing is not well explored and documented. This present study was therefore designed to study the efficacy of these traditional medicines singly and in combinations on excision wounds in Wistar rats. METHODS: At two different intervals (i.e., day 8 and day 16), biomechanical, histological and immunohistochemical (IHC) parameters were assessed at the wound site.  IHC focused on the inflammatory rate by evaluating the level of cytokine, IL1βand the tissue remodeling by studying the activity of myofibroblasts.RESULTS: Rapid epithelization, better remodeling, favorable inflammatory changes and an adequate myofibroblast activity at the wound site was observed in all the treated groups compared to control.CONCLUSION: This study is therefore useful in exploring the mechanism of action of these traditional medicines and providing valuable scientific evidence. KEYWORDS:  Wound Healing, Honey, Ghee, Glycyrrhiza glabra, Nerium indicum